(PR47) Evaluate the Effects of Calcium Hydroxide and Dermaseptin S1 on E. Faecalis and Dental Pulp Stem Cells

Abstract: Endodontic treatments can fail due to persistence of infection. Antimicrobial peptides might be a good alternative to calcium hydroxide for intracanal medication. The objectives of this study are to compare the antimicrobial properties of dermaseptin S1 (DS1) and Ultracal XS against E. faecalis growth, and their compatibility with dental pulp stem cells (DPSC).

Methods: E. faecalis was cultured in the presence or not of Ultracal XS (Ultradent), calcium hydroxide, barium sulfate, propylene glycol or DS1 (10, 50 and 100 µm/mL). The bacterial growth was assessed over 24 h by spectrophotometry. The effect of the different chemicals on shape and growth of DPSCs were evaluated using optical microscope at 24 and 48 h, and MTT assay after 48 h. Data were collected from 3 experiments and subjected to statistical analysis using One-way ANOVA and Tukey’s test.

Results: Ultracal XS, slightly decreased the growth of bacteria, while calcium hydroxide, barium sulfate and propylene glycol have no effect on the growth of E. faecalis. Interestingly, DS1 significantly reduced the growth E. faecalis. The inhibitory effect was present up to 24 h culture, with 50 and 100 ug/ml of DS1. Ultracal XS and its constituents have high toxic effect on DPSC adhesion and growth, while DS1 showed moderate adverse effect only at high concentration (100 µg/ml).

Conclusions: Ultracal XS moderately, while DS1 highly decreased the growth of E. faecalis. The toxic effect of DS1 on DPSCs was moderate compared to Ultracal XS. This study demonstrated the potential use of dermaseptin as an intracanal medication.