Assistant lecturer at Al-Azhar university Al-Azhar university Mansura, Ad Daqahliyah, Egypt
Objective: Our aim was to evaluate the cytotoxicity of MTA versus Biodentine and Endosequence putty extracts at three different observation periods (24 h, 48 h, and 96 h) using the MTT assay. 72 wells in three culture insert plates were used in this study. In addition to MTA, Biodentine and Endosequence putty were evaluated in a fresh state. Passage four of human PDL cells were included in this study. After cell preparation and culturing, an extract of the three tested materials was prepared and cultured with the complete media. In addition to 5×104 cells were implanted into each well 24 h earlier and incubated in a stem cell incubator. The MTT assay was used to evaluate in vitro cytotoxicity, calculating the viable cells by hundred percent using the enzyme-linked immunosorbent assay (ELISA) system and the inverted light microscope was used to reassure their confluence. Evaluation of cell viability was done after three different observation periods (24 h, 48 h, and 96 h), after exposure to the material extracts. Endosequence and Biodentine cements showed no statistically significant differences at all the observation periods. However, MTA cement resulted in a statistically significant difference at all the observation periods but none of them below the cut level. Meanwhile, all the material extracts resulted in a statistically significant increase in cytotoxicity at 24 h, a decrease at 48 h, and finally a little increase in cytotoxicity at 96 h. All the tested materials showed low cytotoxicity and are comparable to the old standard MTA.
