Abstract: Dengue Fever outbreaks in Guatemala are occurring at increased rates. This study examined socioeconomic and environmental factors associated with Dengue in Guatemala. Socioeconomic factors included population density, literacy, internet use, Mayan speakers, economic activity, and attending school. Environmental factors included elevation, temperature, and precipitation. Zero-inflated negative binomial regressions found population density, internet use, attending school, Mayan speakers, and temperature were significantly associated with Dengue. Environmental and socioeconomic factors both relate to Dengue incidence in Guatemala.