PB0090 - Investigating Levels of Inflammatory Biomarkers Between SARS-CoV-2 Vaccinated and Unvaccinated Subjects

Sunday, July 10, 2022

6:30 PM – 7:30 PM

Location: Exhibition

Presentation Format: Virtual

Co-Author(s)

NJ

Nusrat Jabeen, PhD

Associate Professor
University of Karachi
Karachi, Sindh, Pakistan
AA

Anusha Amanullah

Student
Dow University of Health Sciences
Karachi, Sindh, Pakistan
MH

Mushtaq Hussain

Professor
Dow University of Health Sciences
Karachi, Sindh, Pakistan
BK

Bulent Kantarcioglu

Senior Research Office
Department of Molecular Pharmacology & Neuroscience, Loyola University Medical Center, 2160 S. 1st Avenue, Maywood, IL 60153, USA.
Maywood, Illinois, United States

Presenting Author(s)

Fakiha Siddiqui, PhD Candidate

PhD Candidate
Department of Pathology and Laboratory Medicine, Cardiovascular Research Institute, Loyola University Chicago, Health Sciences Division, Maywood, IL, USA.
Maywood, Illinois, United States

Co-Author(s)

JF

Jawed Fareed, n/a

Professor
Loyola University Medical Center
MAYWOOD, Illinois, United States

Background: To curb the threat of COVID-19, vaccines of different forms and shape have been developed and assessed for their efficacy in the last one and a half year. Amongst those Inactivated viral vaccines developed in China, Sinopharm and Sinovac are the most frequently employed vaccines in Pakistan. It has been established that natural infection and certain forms of SARS-CoV-2 vaccine alters the clinical picture of blood.

Aims: In this study we have compared the levels of three inflammatory biomarkers namely PAI-1, D-Dimer and HAI-IgG in the sera collected from SARS-CoV-2 Vaccinated and unvaccinated Subjects.

Methods: Briefly, 80 individuals, each as a cohort of SARS-CoV-2 vaccinated and unvaccinated were recruited with written consent after ethical approval for the study. From each subject 2 ml blood was drawn and plasma was separated to assess inflammatory biomarkers like PAI-1, D-Dimer and HIA IgG by ELISA. Additionally, platelets count were also monitored using automated counter.

Results: Our data show difference in the level of PAI-1, D-Dimer and HIA-IgG between SARS-CoV-2 Vaccinated and unvaccinated subjects. However, the difference was found statistically in significant. Nevertheless, segregating the data based on the nature of vaccination, age and gender of the subjects shows interesting pattern that could be insightful in relation to the clinical outcome of the vaccine efficacy.

Conclusion(s): The findings in this regard could well be of clinical value, especially when the data is stratified with reference to different variables. Therefore, large scale studies are warranted with same, and few additional biomarkers would be of more resolving in relation to the host response against SARS-CoV-2 vaccination.