Assay Development and Screening
Paul Held
Laboratory Manager
Agilent Technologies
Burlington, VT, United States
Over the course of the last 20 years, isothermal nucleic acid amplification tests, such as loop-mediated isothermal amplification (LAMP) have emerged as an important diagnostic tool, not only for clinical applications, but also for food quality control and environmental monitoring. LAMP is an assay technology that has gained traction for its ability to detect nucleic acid sequences under a variety of different conditions without specialized equipment1. While typically run as an end point reaction in PCR tubes using manual observation for positive/negative determinations, the use of colorimetric pH change detection lends itself to automated kinetic monitoring. Here we describe the adaptation of a PCR tube-based assay to microplates and the subsequent use of the Agilent BioTek Synergy Neo2 and Synergy H1 multimode readers to run LAMP assays at low volumes in 384-well microplates.
For Research Use Only. Not for use in diagnostic procedures.
RA44551.5452199074
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