Senior Associate Scientist Gilead Sciences Foster City, CA, United States
Abstract: Small molecule drug plasma bioanalysis using liquid chromatography-tandem mass spectrometry (LC-MS/MS) is routinely used to quantify drug concentrations in nonclinical animal studies. The data derived from plasma quantification provides useful pharmacokinetics information to advance the understanding of small molecule drug candidate(s) to the research discovery teams. In in vivo studies, blood samples are collected, and plasma harvested at designed time points following administration to animals. The plasma bioanalysis includes a labor intensive sample preparation followed by LC-MS/MS analysis. We have introduced an automated plasma sample preparation to enhance sample preparation workflow using the Biomek i7 workstation. The sample preparation – including the preparation of standard curve, quality control samples and unknown sample are prepared within 47 min. The prepared samples are analyzed using by an ultra-high pressure liquid chromatography (UHPLC; Shimadzu LC-30 stack) system that is coupled with Sciex 6500+ triple quadruple mass spectrometer. The Biomek i7 automated workstation demonstrates a robust and reliable sample preparation for plasma derived from a range of nonclinical species. The performance of the automated sample preparation platform was verified by conducting a manual preparation followed by UHPLC-MS/MS analysis. Excellent correlation (goodness of fit, R2 = 0.997) was observed in a linear plot of the concentrations derived from the automated and manual prepared samples from at least ten different studies. The UHPLC-MS/MS has significantly reduced the sample analysis time to approx. 1 minute/sample, cut the cost by using the small size of LC column, and reduced the pollution to environment by cutting the usage of mobile phases. This combination of automated sample preparation workflow and the UHPLC-MS/MS for sample analysis helps increase the bioanalysis throughput, data turnaround time, reduce repetitive injuries from manual pipetting and reduce possible human error.
Scan and Win! Complete as many scavenger hunt questions as possible. You will find scavenger hunt QR Codes to scan on participating posters. Each participating poster in the exhibit hall will have a QR code next to it. For virtual participants, look for the scavenger hunt icon for participating posters.
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Instructions
Each poster participating in the scavenger hunt will have a SCAVENGER HUNT QR code. When that QR code is scanned using the SLAS2022 app, question about the poster will appear. You must answer the question correctly to collect points. BE SURE TO EXPLORE THE POSTER BEFORE SCANNING THE CODE! SLAS will award three prizes to the participants with the most points.
1st Prize - Comp Reg + Hotel/Airfare to SLAS2023 in San Diego
2nd Prize - $50 Starbucks Gift Card
3Rd Prize - $25 AMEX Gift Card
Keep an eye on the leader boards to see who’s at the TOP. Winners will be announced after SLAS2022.
Each participating poster in the exhibit hall will have a QR code next to it. For virtual participants, look for the scavenger hunt icon for participating posters.
