Quality attributes and characterization of gene vectors using light scattering

Tuesday, October 18, 2022

1:30 PM – 2:30 PM ET

Location:360 Stage #1, Exhibit Hall A&B

Presented By

Partner Presentation Speaker(s)

John Champagne, PhD

Senior Application Scientist & Northeast Regional Manager
Wyatt Technology
Goleta, California

Handouts

Description: The recent success of gene therapy owes greatly to the delivery vehicles used, such as lentiviruses (LV), adenoviruses (AdV), adeno-associated viruses (AAV), and lipid nanoparticles (LNP). Quantifying quality attributes of these gene vectors is of increasing importance as the gene therapy products enter into the late development stages and QC environment. In this presentation, we demonstrate the use of three analytical techniques. Batch dynamic light scattering (DLS) is for rapid screening (size and concentration), size exclusion chromatography coupled to multi-angle light scattering (SEC-MALS) for multiple AAV CQAs quantification (capsid particle number, empty/full ratio, and percentage of aggregates), and field-flow fractionation (FFF) with MALS for quantifying AAV aggregates and extended characterization of large gene vectors.

Learning Objectives:

Participant will understand how to quantify AAV multi-CQAs in one single SEC-MALS run.
Participant will understand quantification of LNP payload, size distribution, concentration, morphology, and stability by SEC/FFF-MALS.
Participant will understand extended characterization of lentivirus and adenovirus by FFF-MALS.