The nature of expression based peptides leads to substitution variants that may differ by only a single residue from the target sequence, and present a challenge to scaling of effective chromatographic separation. We will discuss the use of column temperature control for the separation of short-chain peptides, and the potential gains in fraction purity that can be realized by increasing resolution. While small molecule purification at similar scale has typically eschewed the use of column temperature control, the typical yield, loading requirements, and retention factors for biomolecules will be examined and contrasted with prior art.
Learning Objectives:
Understand the role LC purification plays in the scale up of drug candidates.
Develop a basic understanding of the challenges that differentiate small molecule versus biomolecule purificiation.
See the effects of temperature control on the retention of short chain peptides.
