The presentation will start with an overview of EV biology and why they are emerging as a promising therapeutic and diagnostic platform. We will describe the complexity of EV in terms of heterogeneity and cargo selection and how it affects biomarker development. Then, we will go over different isolation methodologies including ultracentrifugation, size exclusion, precipitation, filtration, immunoaffinity, and charge-based approach, and discuss the advantages and disadvantages of each methodology. Next, I will go over different ways to measure EVs including nanoparticle tracking analysis, electron microscopy, nano view, FACS, Atomic force microscopy, and intact EV immunoassays, and discuss the advantages and disadvantages of each methodology. We will then discuss the challenges in identifying the right normalization strategy for EV-based biomarkers and in the analytical validation of EVs based approach. Finally, I will give a quick overview of current and upcoming EV-based diagnoses.
Learning Objectives:
What are EVs and why they are a promising biomarker platform
what are the available methods to work with EVs and what is the advantage and disadvantage of each methodology
Why I believe that Immuneaffinity based isolation provides the most promising approach to fully uncover the EV promise
