539.1 - Characterization of a Cleavable Fusion of Human CYP24A1 with Adrenodoxin Reveals the Variable Role of Hydrophobics in Redox Partner Binding
Monday, April 4, 2022
9:40 AM – 9:50 AM
Room: 113 C - Pennsylvania Convention Center
Introduction: This session will feature a lecture by the 2022 winner of the Bernard B. Brodie Award as well as talks from the authors of the two best papers of 2021 from the journal of Drug Metabolism and Disposition who received the James R. Gillette Awards in pharmacokinetics (transporters) and drug metabolizing enzymes. The session will also include abstract-based oral presentations from graduate students, post-bacs, and postdocs. Submit your abstract to the Drug Metabolism and Disposition topic categories below to be considered for the platform session.
Natalie Jay (University at Buffalo), Sean Duffy (University at Buffalo), Jeff Tu (University at Buffalo), Fernando Estrada (University at Buffalo)
The improper maintenance of the bioactivated form of vitamin-D (1α,25(OH)2D) may result in vitamin-D insufficiency and therefore compromise the absorption of dietary calcium. A significant regulator of vitamin-D metabolism is the inactivating function of the mitochondrial enzyme cytochrome P450 24A1 (CYP24A1). In humans, CYP24A1 carries out hydroxylation of carbon-23 (C23) or carbon-24 (C24) of the 1α,25(OH)2D side chain, eventually resulting in production of either an antagonist of the vitamin-D receptor (C23 pathway) or calcitroic acid (C24 pathway). Despite its importance to human health, the human isoform (hCYP24A1) remains largely uncharacterized due in part to the difficulty in producing the enzyme using recombinant means. In this study, we utilize a cleavable fusion with the cognate redox partner, human Adx (hAdx), to stabilizing hCYP24A1 during production. Subsequent cleavage and isolation of active hCYP24A1 allowed for an investigation of substrate and analog binding, enzymatic activity, and redox partner recognition. The redox partner recognition relies on electrostatics, specifically Asp-72 and Asp-76, of hAdx. However, we also demonstrate involvement of a non-polar contact between Leu-80 of hAdx and a non-conserved proximal surface of hCYP24A1. Using a combination of functional assays, spectral binding data, and nuclear magnetic resonance (NMR) spectroscopy, we determine that the contact at Leu-80 is distance-dependent; shortening the length of this residue (L80V) results in enhanced binding between the CYP-Adx complex as measured by protein NMR, promotes more binding of 1α,25(OH)2D, yet unexpectedly results in decreased catalysis. The same mutation has a negligible effect on rat CYP24A1 (a C24-hydroxylase), indicating the presence of a species-specific requirement that may correlate with differences in regioselectivity of the reaction. We combine these findings with an NMR characterization of the hAdx dimer to inform a model of a 1:2 hCYP24A1:hAdx complex.
