790.2 - Identification of Signaling Pathways and Phase Separating Domains that Drive Cajal Body Formation

Madelyn Logan (University of Mississippi Medical Center), Douglas McLaurin (University of Mississippi Medical Center), Katheryn Lett (University of Mississippi Medical Center), Michael Hebert (University of Mississippi Medical Center)

Cajal bodies (CBs) are membraneless subnuclear domains that contribute to the biogenesis and maturation of ribonucleoproteins (RNPs) which take part in fundamental cellular activities such as translation, pre-mRNA splicing, and telomere maintenance. Partitioning of CBs occurs through liquid-liquid phase separation (LLPS) where the CB marker protein, coilin, is thought to scaffold the CB. However, the formation of CBs and the regulation of CB number has not been fully elucidated. Disease states such as cancer and preeclampsia have altered CB expression, thus additional insight into CB condensation would provide an important cellular model system that could be used to investigate these and other diseases. We hypothesize that the post-translational modification (PTM) of coilin regulates CB formation and number by influencing the LLPS of coilin via residues in its intrinsically disordered region (IDR). Here we show, for the first time, a cycloheximide inducible model of CB formation in fibroblasts without increasing coilin concentration. We are also able to induce CB formation by inhibiting kinase GSK3β with indirubin, as well as abolish CB formation by inhibiting phosphatases PP1 and PP2A with okadaic acid. Lastly, we have identified two phase-separating domains in coilin’s IDR using the phase separation predicting software ParSe. Deletion of each domain prevents CB formation in HeLa cells but does not prevent coilin’s self-interaction. Collectively, these data will be able to help us understand the role of coilin’s scaffolding capabilities via LLPS mediated CB condensation, as well as provide an important cellular model system that can be used to investigate CB functionality in disease states where CB formation is dysregulated.

Funding for this research is supported by the Intramural Research Support Program of The University of Mississippi Medical Center.