(640.3) Comprehensive Characterization of Biological Properties of Human Urine-Derived Stem Cells

Poster Board Number: C158
Introduction: AAA has separate poster presentation times for odd and even posters.
Odd poster #s – 10:15 am – 11:15 am
Even poster #s – 11:15 am – 12:15 pm

Lubos Danisovic (Faculty of Medicine, Comenius University, National Institute of Rheumatic Diseases), Martina Culenova (Faculty of Medicine, Comenius University, National Institute of Rheumatic Diseases), Zuzana Novakova (Faculty of Medicine, Comenius University), Andreas Nicodemou (Faculty of Medicine, Comenius University), Michaela Debreova (National Institute of Rheumatic Diseases), Veronika Smolinksa (Faculty of Medicine, Comenius University), Sona Bernatova (Faculty of Medicine, Comenius University), Ivan Varga (Faculty of Medicine, Comenius University, National Institute of Rheumatic Diseases), Stanislav Ziaran (Faculty of Medicine, Comenius University, National Institute of Rheumatic Diseases)

Mesenchymal (stromal) stem cells (MSCs) represent a unique tool in the field of regenerative medicine. However, their harvesting often requires invasive medical procedures. Urine-derived stem cells (UDSCs) display similar properties to MSCs, and their sampling and further processing is non-invasive for the donors. Here, we offer a comprehensive analysis of their biological properties. The goal of this study was to analyze their morphology, stemness, differentiation potential and cytokine profile. We have successfully isolated UDSCs from 25 urine samples. First colonies emerged up to 9 days after the initial seeding. Cell doubling time was 45 ± 0.24 SD, and when seeded at the density of 100 cells/cm2, they formed 42 ± 6.5 SD colonies within 10 days. Morphological analyzes revealed that two distinct types of the cell populations have been present. The first type had a rice-grain shape and the second one was characterized by a polyhedral shape. All examined UDSCs expressed typical MSC-like surface markers, CD73, CD90 and CD105. Moreover, conditioned media from UDSCs were harvested, and cytokine profile has been evaluated showing a significantly higher secretory rate of IL-8, IL-6 and chemokines MCP-1 and GM-CSF. We have also successfully induced human UDSCs into chondrogenic, osteogenic and myogenic cell lineages. Our findings indicate that UDSCs might have immense potential in the regeneration of the damaged tissues.

This publication is the result of the project implementation CEMBAMamp;mdash;Centre for Medical Bio-Additive Manufacturing and Research, ITMS2014+: 313011V358 supported by the Operational Programme Integrated Infrastructure funded by the European Regional Development Fund.