(827.1) Mechanical Tension-induced Vascular and Alveolar Morphogenesis during Lung Regeneration

Poster Board Number: D35

Akiko Mammoto (Medical College of Wisconsin), Tendai Hunyenyiwa (Medical College of Wisconsin), Priscilla Kyi (Medical College of Wisconsin), Kathryn Hendee (Medical College of Wisconsin), Kienna Matus (Medical College of Wisconsin), Tadanori Mammoto (Medical College of Wisconsin)

Mechanical forces altered after unilateral pneumonectomy (PNX) control post-PNX vascular and alveolar morphogenesis. This study aims to understand the mechanosensitive mechanism of lung vascular and alveolar regeneration in the post-PNX mouse lungs. Vascular formation is stimulated at the subpleural region in the remaining mouse lung after unilateral PNX, where mechanical stretch is enhanced, while inhibition of mechanical tension by insertion of prosthesis attenuates these effects. The focal adhesion protein, paxillin senses mechanical stretch after PNX and the levels of paxillin are upregulated in SPB-positive alveolar epithelial type II (ATII) cells and endothelial cells (ECs), which stimulates the expression of angiogenic factor angiopoietin-1 (Ang1) in ATII cells and Ang2 in ECs to induce EC directional motility to the subpleural region. Cellular stretching increases the expression of paxillin, which induces expression of Ang2 in ECs and Ang1 in ATII cells in vitro. Post-PNX lung growth and vascular formation toward the subpleural region are suppressed in Pxnfl/fl-Sftpc-CreERT2 mouse lungs, in which paxillin expression is attenuated in ATII cells. Structure of lamellar bodies that control surfactant homeostasis is also disrupted in post-PNX Pxnfl/fl-Sftpc-CreERT2 mouse lung ATII cells. These results suggest that cooperative action of mechanosensitive paxillin signaling in ECs and ATII cells mediates vascular and alveolar epithelial formation during post-PNX lung growth. 

Support or Funding Information

NIH R21AG054830, R01HL139638, R21AG062893, R01HL142578, AHA 18TPA34170129

NIHamp;nbsp;R21AG054830,amp;nbsp;R01HL139638, R21AG062893, R01HL142578, AHAamp;nbsp;18TPA34170129