Presenting Author Johannes Gutenberg University Mainz, Rheinland-Pfalz, Germany
The urinary bladder is enlarged in all models of type 1 and several of type 2 diabetes (doi 10.1002/nau.23786). We have previously reported that such enlargement is correlated to insulin, but not glucose levels in fructose-fed rats (doi 10.1096/fasebj.2020.34.s1.00542). Because this was surprising, we have now repeated that study and expanded our analysis to other models of diabetes and obesity.
Five studies were performed in models of type 2 diabetes and obesity, one in fructose-fed rats (n = 16), one each in ZSF1 rats at age 20 and 28 weeks (a cross between a female Zucker Diabetic Fatty and a male spontaneously hypertensive SHHF rat; n = 30 and 45, respectively), and two in mice on a high-fat diet (n = 23 and 24, respectively). Bladder weight at study end and blood insulin concentrations were subjected to linear correlation analysis based on individual animal data, and data expressed a squared Pearsson correlation coefficient (r2).
Animals in the diabetic/obese groups were hyperinsulinemic, whereas bladder enlargement was observed in both studies with ZSF1 rats, the old but not the new in fructose-fed rats, and in neither study with mice on a high-fat diet. Bladder weight and insulin levels were not correlated in the new study in fructose-fed rats or any of the other four studies; the numerical correlation coefficient was negative in two of these studies. When data from all six studies were pooled, a very weak inverse correlation was observed (r2 0.0682, descriptive p-value 0.0094; Figure 1). We conclude that, contrary to our previous observation, bladder weight is not positively correlated with insulin levels in rodent models of type 2 diabetes and obesity.
This work was funded in part by Tamp;Uuml;BITAK-SBAG 118S443 and 119S769 (to EAI), Conacyt Mexico 252702 (to DC), and Deutsche Forschungsgemeinschaft XI 139/2-1 (to NX), LI-1042/5-1 (to HL) and Mi 294/10-1 (to MCM). Some underlying studies were performed or funded by Sanofi-Aventis (identified as amp;ldquo;Hoechstamp;rdquo;) for purposes unrelated to this manuscript.
Correlation of insulin levels and bladder weight in the pooled data from all studies and in three studies shown as examples.
