Session: 556 APS Endothelial Cell Biology in Health and Disease Poster Session
(556.28) Exercise Training Alleviates Alzheimer’s Disease-Associated Aortic Endothelial Dysfunction
Sunday, April 3, 2022
10:15 AM – 12:15 PM
Location: Exhibit/Poster Hall A-B - Pennsylvania Convention Center
Poster Board Number: E120
Junghoon Lee (University of Houston), Aliasghar Zarezadehmehrizi (University of Houston), Junyoung Hong (University of California-Davis), Jonghae Lee (The University of Texas MD Anderson Cancer Center), Yoonjung Park (University of Houston)
Backgrounds: A strong association between cardiovascular disease and Alzheimer’s disease (AD) has been reported, but its possible mechanism has been unknown. We have tested whether aortic endothelial function in AD is impaired and investigated the protective effect of exercise training (EX) on endothelial function in aorta of AD mice.
Methods: Wild type (C57BL/6, WT) and AD (APP/PS1dE9, AD) mice underwent treadmill training (WT-EX and AD-EX) or remained sedentary for 12 weeks. The expression of total-and phosphorylated-endothelial nitric oxide synthase (t-eNOS and p-eNOS), beta-amyloid, and superoxide dismutase 2 (SOD2) were quantified by Western blot analysis.
Results: t-eNOS and p-eNOS expressions in AD were decreased compared to WT but EX increased both t-eNOS and p-eNOS in AD (P lt; 0.01 and P = 0.09). Beta-amyloid levels in AD were higher compared to WT but EX decreased it (P lt; 0.05). SOD2 levels in AD were increased by EX (P lt; 0.05) whereas there was no change in WT.
Conclusion: Regular aerobic exercise alleviates e-NOS-mediated endothelial dysfunction in aorta of AD mice possibly via reduced beta-amyloid and increased SOD2 levels.
Support or Funding Information
This study was supported by University of Houston College of Liberal Arts and Social Science Research Progress Grant and Project Completion Grant, American College of Sport Medicine Foundation Doctoral Student Research Grant 17-00587, and National Heart, Lung, and Blood Institute Grant R01- HL-136952 (to Dr. Yoonjung Park).
Fig. 1. Effect of exercise training on the expression of total-and phosphorylated-endothelial nitric oxide synthase (t-eNOS and p-eNOS) in aorta of Alzheimer’s disease (AD) mice. WT, wild type; EX, exercise training;. Values are means ±SE; n = 10/group. *P < 0.05; **P < 0.01.; Fig. 2. Effect of exercise training on the expression of beta-amyloid and superoxide dismutase 2 (SOD2) in aorta of Alzheimer’s disease (AD) mice. WT, wild type; EX, exercise training. Values are means ±SE; n = 10/group. *P < 0.05.