UHRF1 and UHRF2 proteins are multi-domain epigenetic proteins. These two proteins share many similarities in sequence and structure. Previous research in our lab has shown that these proteins bind to H3K9me3 tail peptides. The purpose of our study is to measure UHRF1 and UHRF2 protein interactions with nucleosomes, which are more biologically relevant binding partners. These experiments will be conducted by electrophoretic mobility-shift or nucleosomes pull-down assays. The procedures will be optimized using H3K9me3 and unmodified nucleosomes, with the goal of using the assay to identify novel binding partners for UHRF1 and UHRF2.
