Session: SSASAS: Physiology II

421 - Genomic Effects of Arginine on Cell Behavior of Conceptus Trophectoderm in Ungulates

Friday, July 16, 2021

3:15 PM – 3:30 PM EST

Location: Kentucky International Convention Center (KICC), Ballroom D/E

Presenting Author(s)

Sudikshya Paudel, MS

Graduate Student
Department of Animal Science, North Carolina State University
Raleigh, North Carolina

PDF Slides and Audio

Author(s)

HY

Hongyao Yu

Postdoctoral Fellow
Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences
Research Triangle Park, North Carolina
TW

Tianyuan Wang

Integrative Bioinformatics Support Group, National Institute of Environmental Health Sciences
Research Triangle Park, North Carolina
FB

Fuller W. Bazer

Professor
Department of Animal Science, Texas A&M University
College Station, Texas
Guoyao Wu, n/a

Professor
Department of Animal Science, Texas A&M University
College Station, Texas
RB

Robert Burghardt

Professor
Department of Veterinary Integrative Biosciences, Texas A&M University
College Station, Texas
GH

Guang Hu

Epigenetics and Stem Cell Biology Laboratory, National Institute of Environmental Health Sciences
Research Triangle Park, North Carolina
XW

Xiaoqiu Wang, n/a

Assistant Professor
Department of Animal Science, North Carolina State University
Raleigh, North Carolina

Hatched ungulate (e.g., pigs, sheep and other ruminants) blastocysts undergo dramatic morphological transitions from spherical to tubular to filamentous forms to conceptuses (embryo/fetus and associated extraembryonic membranes) before implantation. L-Arginine (Arg), a conditionally essential amino acid, is required for this process to activate the mTOR cell signaling pathway to induce proliferation of both porcine and ovine conceptus trophectoderm cells. However, the genomic effects of arginine on trophectoderm cells is unknown. RNA-seq was used for a comparative transcriptome analysis of porcine and ovine trophectoderm cells to further understand effects of Arg on regulation of metabolism in trophectoderm cells. An established porcine trophectoderm (pTr) cell line isolated from Day12 porcine conceptuses, as well as an established ovine trophectoderm (oTr) cell line isolated from Day15 ovine conceptuses were used to determine response to Arg at the physiological concentration of 0.2 mM in a 48-h culture. In pTr cells, a total of 2,723 differentially expressed genes (DEG; 1,482 up and 1,241 down) were identified in response to Arg. In oTr cells, a total of 5,369 DEG (2,819 up and 2,550 down) were detected. Comparison analyses showed that the Arg-treated pTr and oTr transcriptomes share 873 common DEG (273 up and 342 down). Canonical pathway analyses identified the top enriched pathways in both pTr and oTr cells, including activation of actin cytoskeleton signaling, adrenomedullin signaling, and IGF-1 signaling; and inhibition of cell cycle G2/M checkpoint regulation, and p53 signaling. In response to Arg, pathways associated with cholesterol biosynthesis, and estrogen-mediated S-phase entry were exclusively activated in the pTr cells; whereas interferon signaling, ephrin receptor signaling, and integrin signaling were specifically activated in the oTr cells. Results from this study advance understanding of mechanisms responsible for elongation of ovine and porcine conceptuses and enable the rational design of future experiments.