Introduction: Compared with other commercially used lasers, the coagulation layer of the novel 450nm laser is thinner, and the thickness of the coagulation layer is the key factor affecting the wound healing. In this study, we investigate whether the novel 450nm 200W laser system is superior to the classic transurethral resection of the prostate (TURP) for wound healing in beagles. Methods: Twenty-two 6-8-year-old male beagles were treated with TURP or blue laser vaporization of the prostate (BLVP), and the prostate wound was observed using cystoscopy 3 h, 1, 2, 3, and 5 weeks after the operation (two beagles in each group). Two elderly beagles without the operation were used as the normal controls. After the cystoscopy examination, the prostate was collected and fixed for hematoxylin and eosin (H&E) and immunofluorescence staining, and the dynamic process of wound healing was observed under the microscope. Results: The urethras of the prostates under cystoscopy in the BLVP groups were healed three weeks after surgery, while in the TURP group, they were healed five weeks after surgery. The H&E staining confirmed that the coagulation necrosis layer in the TURP group was thicker than that in the BLVP group, and it took more time to remove the coagulation necrosis after surgery. The change in the macrophage polarity in the BLVP group was also earlier than that in the TURP group. Conclusions: The new 450nm 200W laser was superior to TURP for wound healing. The thinner coagulation layer of the 450-nm laser was the primary reason for this process. SOURCE OF Funding: None
