Introduction: Pelvic pain is a complex phenomenon for which many women seek treatment from urologists. If pelvic floor trigger point injections are not taught in residency or fellowship, a urologist may not feel comfortable offering this treatment therefore referring the patient to another specialty or urologic subspecialist. This video aims to be an all-encompassing video one can watch to familiarize oneself with the pelvic floor anatomy, positioning, specific distances, and pitfalls for transvaginal pelvic floor injections by the use of animations, cadaveric model, and needles into specific proposed injection sites. Once watching this video one will feel comfortable performing this therapy. Methods: A single female cadaver was dissected by the authors to identify specific pelvic structures relevant to pelvic floor trigger point injections. A video recording was taken demonstrating a standard pelvic floor injection template as described by Gupta et al. Spinal needles were placed into the proposed area of injection. Voice over and picture in picture cartoon animations were used for enhanced visual aid. Results: The location of the spinal needles in the cadaver model shows exact placement into the pelvic floor muscles that are being targeted for pelvic floor injections. This video demonstrates the close proximity of the obturator nerve and vessels 1-o’clock injection sites. It also highlights the close proximity of injection in relation to the sacrospinous ligament and pudendal vessels. Conclusions: Pelvic pain is a complex phenomenon for which many women seek treatment from urologists. If pelvic floor trigger point injections are not taught in residency or fellowship, a urologist may not feel comfortable offering this treatment therefore referring the patient to another specialty or urologic subspecialist. Once watching this video one will feel comfortable performing this therapy. SOURCE OF Funding: None