Abstract: Modifying the Standard Topical Bioassay: an Innovative Method for New Modes of Action
Topical dose response applications are an essential step in the process of assessing potential active ingredients for viability in mosquito control. The standard topical application methodology, utilizing Burkard and Hamilton micro-applicators, has long been used for topical bioassays and applies a known dose of insecticide directly to the exoskeleton of insects, thereby ensuring exposure to a specific amount of compound.
Development work to identify and design next-generation mosquito control products featuring new active ingredients, new modes of action, and new formulation science has laid the groundwork for innovation in dose-response methodologies. This presentation will explore a novel topical dose-response method developed and evaluated in Clarke’s bioassay lab using Drummond Nanojects. With this method and equipment, nanoliter-sized droplets of prototype formulations, diluted with formulation inerts, can be applied onto the mosquito cuticle, allowing droplet behavior and effect on the mosquito to be observed in new ways compared to the traditional methods. This innovation in topical dose-response testing for new modes of action or new active ingredient products for mosquito control allows for more rapid prototype assessments and provides better predictability of droplet penetration and performance in the field.
