Abstract: Aedes aegypti (Linnaeus, 1762) is the most important arbovirus vector with wide distribution worldwide. In our study, we evaluated the spatio-temporal patterns of kdr mutation frequencies in relation to sociodemographic and environmental variables. The genotypic and allelic frequencies of kdr mutations in Nuevo Leon, Veracruz and Yucatan, Mexico were monitored in 2020 and 2021, as well as the use of insecticides. We report 23 of 27 possible haplotypes, being the triple mutated (L410/L410-I1016/I1016-C1534/C1534) the most frequent, followed by the homozygous mutated haplotype in 1016 and 1534 in combination with the heterozygous genotype in 410. (V410/L410-I1016/I1016-C1534/C1534). According to the results, it was determined the influence of temperature, rainfall, number of inhabitants, type of locality and the presence of other kdr mutations on the frequency of mutated alleles. It was observed for samples recollected in 2020 that mutated allele C1534 frequency influenced frequency of mutated alleles I1016 and L410, while the frequency of the mutated allele L410 is influenced by the number of inhabitants in localities. In samples of 2021 we report that frequency of the mutated allele C1534 was influenced by the frequency of the allele L410, while the frequency of L410 was influenced by temperature and the average annual rainfall of the locality. In addition to this, spatial prediction IDW maps were made using the frequency of mutations obtained for both years, which were contrasted with the history of insecticide application in the collection sites, as well as the percentage of mortality obtained from bioassays from the CDC with diagnostic dose of deltamethrin.
