Abstract: The need for the development of new active ingredients for the control of adult mosquitoes is an ever-growing concern for those that deal with pyrethroid resistance, which poses one of the more serious threats to integrated pest management programs throughout the world. Valent BioSciences Mosquito Adulticide project has been in the works for many years and the development of this new product represents the first novel adulticide mode of action in the US in nearly 50 years. An Experimental Use Permit (EUP) was approved by the US EPA for aerial applications of the new adulticide targeting pyrethroid-resistant mosquitoes. Evaluations of the three active ingredients have been ongoing and currently we are investigating the efficacy of aerial applications with caged mosquito field trials according to the EPA requirements. The test design layout, data collection, droplet analysis methods, and flight offsets used for successful evaluations will be covered. Additionally, a review of equipment set-up, calibration methods and spray parameters used for every trial conducted in 2022 will be presented.
