Abstract: Asian tiger mosquitoes were detected in tire yards in Wisconsin in 2017. Was this cause for alarm and indication of imminent invasion and establishment, or an observation of a transient, seasonal invasion? Using lab-reared mosquitoes in induced diapause, some discarded unwanted tires, and temperature and humidity monitors, we have identified conditions that make tires into cozy overwintering dens for Aedes albopictus - even through the extreme cold winter conditions experienced in Wisconsin and Illinois, USA. Tires are well known as an oviposition site, supportive larval habitat, and mode of transport for this species. We show that tires provide a measure of insulation from cold temperatures. Additionally, we found that tires which are also covered in snow provide insulation for overwinter survival of Aedes albopictus eggs. These findings have important implications for successful establishment of this species, particularly in light of climate projections that suggest decreasing likelihood of deep snow cover.
