306 - Isolation and characterization of bacteria from populations of Aedes aegypti (Diptera: Culicidae) and their role associated with susceptibility to the insecticides permethrin and deltamethrin.

Aedes aegypti mosquito (Diptera: Culicidae) is a mosquito that transmits viral diseases such as dengue, chikungunya, Zika, and yellow fever. Insecticides are one of the most used controls to limit the development and distribution of the mosquito. The indiscriminate use of these chemical agents has triggered resistance. The manipulation of the mosquito microbiota is a promising field for the development of new vector control strategies. Thus, the aim of the study was to characterize key bacteria for the survival of mosquitoes against exposure to pyrethroid insecticides (permethrin and deltamethrin) in populations of Ae. aegypti. Populations of Ae. aegypti from the locality Umán, Yucatán, Mexico was used. Suppression of the microbiota with antibiotics (penicillin/streptomycin and gentamicin) was performed. For the isolation of bacteria was performed using soy trypticase medium (35 ± 2ºC) and its identification by MALDI-TOF. Bacteria were selected for their growth in M9 medium added with deltamethrin and permethrin to assess the response to insecticides. The selected bacteria were supplemented to larval populations of Ae. aegypti New Orleans with microbiota suppression and then exposed to insecticides using CL90. Enterobacter, Klebsiella and Staphylococcus genera were found in the populations. It was determined that the isolated species Enterobacter cloacae and Staphyloccus epidermidis could grow in media containing insecticides. When supplementing the species Klebsiella oxytocia to populations with modified microbiota, there were no significant differences (P< 0.05) in the survival of the population against the evaluated insecticides. These results are essential for a better understanding of mosquito physiology and the role of bacteria in response to insecticides.