272 - miRNomics universe of Aedes aegypti (L) (DIPTERA: CULICIDAE)

The microRNAs (miRNAs) are small single-stranded endogenous RNAs with a length between 21 and 25 nucleotides and these regulate the translation of proteins by hybridization with messenger RNAs. To date, its existence and physiological role have been reported in organisms of all kingdoms. The microRNAs participate by performing multiple and varied functions. To date, it has been shown that miRNAs in mosquitoes play important roles in various biological processes such as blood digestion, oviposition, sexual differentiation, resistance to insecticides and infection with pathogens. At this moment, microRNAs from 33 insect species have been reported in the miRBase database, with a total of 3,857 microRNAs. In this work we describe the battery of microRNAs with exclusive expression of diverse biological approaches that had at least 10 reads and 5 folds of presence. Now we know microRNAs with an exclusive expression in males (10), females (7), adults (45), eggs (13), females fed and sacrificed at 24 (11), 48 and 72 hours. In the present study we report for the first time the mirnomic fingerprint of diverse biological approaches [life stages (eggs and adults), sex (males and females) and the postprandial chronology (0, 24, 48 and 72 hours after feeding)] of Ae. aegypti. The differential expression pattern of the miRNAs specific and shared in stages of life plays a critical role in the physiology of Ae. aegypti. A better understanding of the functions of these miRNAs will have a great implication for the effective control of the population of vectors and, therefore, to interrupt the transmission of diseases caused by pathogens such as Dengue, Zika, Chikungunya, Mayaro and Sepik viruses.