Abstract: The Northeast Regional Center for Excellence in Vector-Borne Diseases (NEVBD) pesticide resistance monitoring program was developed in 2019. The goal of the program is to support and expand capacity across the Northeast and Mid-Atlantic regions to collect baseline resistance data and monitor emerging resistance in mosquito populations. The NEVBD has developed a specimen submission and testing system, adult topical, larval bioassays and mechanism testing in response to regional needs. In addition, we have conducted onsite resistance monitoring training and developed materials to assist municipalities in collecting and testing mosquitoes. From 2019-2022 agencies directly submitted Aedes albopictus and Culex pipiens for larvicide and adult bioassay testing. Mosquitoes were submitted from 11 of 13 states in NEVBD’s catchment area including larval assay requests from 26 agencies spanning 8 states and adult bottle bioassay requests from 20 agencies spanning 9 states. NEVBD and partnering agencies have identified moderate-high resistance to pyrethroids in several locations and emerging resistance to organophosphates in the mid-Atlantic coastal region. In 2022 we embarked on a more thorough investigation of Cx. pipiens pyrethroid and organophosphate resistance in the lower Chesapeake Bay area.