79 - Bioassays and biochemical testing, a comprehensive IR approach

Abstract: Insecticide resistance monitoring is an important part of a comprehensive integrated mosquito management program. At the Lee County Mosquito Control District located in Lee County, Florida, the insecticide resistance program has progressed over the years to include larval bioassays, CDC bottle bioassays, cage trials, and biochemical assays. Larval bioassays are conducted on field-collected larvae of five species of mosquitoes (Aedes aegypti, Aedes alboptictus, Aedes taeniorhynchus, Culex quinquefasciatus and Culex nigripalpus) to monitor the continued efficacy of four different larvicide active ingredients. Bottle bioassays are conducted on adult mosquitoes raised from field-collected larvae of six different species of mosquitoes using four different adulticide active ingredients to monitor for the development of resistance. Field cage trials are conducted following bottle bioassay results to determine the practical resistance level and biochemical assays are conducted to determine resistance mechanisms. A detailed overview of the insecticide resistance monitoring program at the district will be provided.