225 - Microleakage and Antimicrobial Properties of Experimental Antibacterial Fluoride-Releasing Dental Sealants

Purpose: The purpose of this study was to investigate the antimicrobial properties and microleakage of experimental antibacterial fluoride-releasing sealants.

Methods: Two commercial non-antibacterial sealants Clinpro™ (3M ESPE) and Ultradent XT Plus™ (Ultradent) were used as Control-1 and Control-2. Two experimental sealants (Exp-1, Exp-2) were prepared by adding 10% of a synthesized antibacterial fluoride-releasing monomer C16DC2DMA to the two controls, respectively. A microleakage test for all four groups was conducted on extracted teeth using a dye-penetration method. A S. mutans biofilm assay was conducted on all groups using a direct contact assay and drop-plating. Two-factor linear mixed models with random effects for tooth IDs were used to analyze differences in microleakage scores between treatment groups while accounting for repeated measures on each tooth. Pairwise comparisons were made between each treatment group using this mixed model and Wald tests.

Results: None of the microleakage scores were significantly different between pairs of treatment groups(p>= .071) but the two experimental sealants showed significant reduction (by 3 to 7 orders) in biofilm CFU when compared with controls.

Conclusion: The synthesized antibacterial fluoride releasing monomer C16DC2DMA can be used to generate antibacterial sealants with significant biofilm inhibition effect.