Caries
Effect Of SDF On Lactobacillus Acidophilus Associated With ECC
TEJASWI SINGANA, MDS
Pediatric Dental Resident
Nova Southeastern University, Fort Lauderdale, FL
Nova Southeastern University
Davie, Florida, United States
Judith R. Chin, n/a
Pediatric Residency Program Director
Nova Southeastern College of Dental Medicine
Nova Southeastern University College of Dental Medicine
Fort Lauderdale, Florida, United States
Purpose: The main purpose of this study is to investigate the effect of SDF on Lactobacillus acidophilus associated with early childhood caries.
Materials and
Methods: This study is an in vitro experimental study which evaluated the antibacterial effect of SDF (38%) using both disk diffusion assay and minimum inhibitory concentration (MIC) assay. In disk diffusion assay, the antibacterial effect of SDF (38%) was evaluated and compared to that of fluoride varnish (5%), chlorhexidine (positive control group) and Phosphate Buffered Saline (PBS: negative control group) on two different concentrations of L.acidophilus (1 × 106/plate or 1 × 108/plate). The experiment was performed in quadruplicates for each studied group (4 well/group) and each experiment was repeated three times.
Results: SDF (38%) demonstrated higher inhibitory effect compared to Chlorhexidine. MIC of SDF was higher compared to chlorhexidine suggesting that chlorhexidine has more antibacterial effect at lower concentrations.
Conclusion: This study demonstrated the inhibitory effect of SDF on the growth of L. acidophilus, indicating that SDF possess sufficiently efficient antibacterial property and MIC of SDF is higher when compared to chlorhexidine which shows chlorhexidine is more effective antibacterial agent compared to SDF in lower concentrations. This study gained a mechanistic insight into the microbiological impact of SDF in arresting the L. acidophilus-associated ECC and discovered a new beneficial antibacterial efficacy of SDF