(PR71) Impact of Genetic Polymorphisms in the Suppressor of Cytokine Signaling 1 in the Development of Persistent Apical Periodontitis in Brazilians

Objective: This study aimed to investigate molecular aspects involved in persistent apical periodontitis (PAP) and their impacts on the individual response after root canal treatment. Materials and

Methods: Patients with pulp necrosis and apical periodontitis were submitted to root canal treatment with a standardized protocol of materials, including restorative procedures. After one year they were called for a follow-up and 212 patients were selected for this study. Of these, 101 patients had clinical and radiographic signs and symptoms indicative of PAP and 111 indicative of lesion repair. Saliva samples were collected as a source of genomic DNA which was genotyped for suppressor of cytokine signaling 1 (rs243327 and rs33977706) by real-time PCR. All genetic polymorphisms were in Hardy-Weinberg equilibrium. The frequency of genotypes and alleles was evaluated using the odds ratio, chi-squared test, or Fisher's exact tests (p < 0.05).

Results: For SOCS-1 there was no association between genotype distribution (rs243327 p=0.0788/rs33977706 p=0.0789) nor between allelic distribution (rs243327 p=0.12/rs33977706 p=0.0889) in the additive model. However, in the recessive model analysis, there was an association for rs243327 (p = 0.0299) and rs33977706 (p=0.031) with lesion repair.

Conclusion: These results reinforce the influence of host response to root canal treatment, since genetic factors are associated with the development and repair of apical periodontitis.