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(PR43) The Differential TIMP-1 and -2 Expression in Pulp Fluid Serves as a Diagnostic Marker Reflecting the Precise Pulpal Status During Inflammation

Thursday, May 4, 2023

1:00 PM – 4:00 PM CT

Presenter(s)

JS

Jooyoung Song, D.D.S.

Resident
University of California, Los Angeles
Los Angeles, California, United States

Co-Author(s)

Rucha Patil, B.D.S.

Advanced Clinical Trainee, Endodontics
University of California, Los Angeles
Los Angeles, California, United States
RK

Reuben Kim, D.D.S., Ph.D.

Professor and Chair of the Section of Restorative Dentistry
University of California, Los Angeles
Los Angeles, California, United States
IC

Insoon Chang, D.D.S., M.S., Ph.D.

Assistant Professor
University of California, Los Angeles
Los Angeles, California, United States
LM

Laurel Martinez, D.M.D., M.A.

Endodontist
University of California, Los Angeles
El Monte, California, United States

Introduction: A proper development of treatment plan critically depends on pulpal diagnosis reflecting the accurate status of the pulp. In this study TIMP1 and TIMP2 have been identified as potential biomarkers in pulpal fluid (PF) that may be utilized to obtain precise diagnosis of pulpitis. TIMPs are key regulators of the MMPs and associated with tissue degradation and remodeling. Employed together with clinical examinations, TIMPs may serve as potential diagnostic tools to identify different stages of pulpitis.

Objective: To explore the TIMPs expression in the PF and pulp tissue of sound, reversible pulpitis (RP) and irreversible pulpitis (IP) and identify their role in inflammation. Materials and

Methods: Extracted teeth were sectioned on the occlusal, split, and pulp tissue and PF were collected: Sound dentin (n=3), RP (n=3), and IP (n=3). Groups were determined by clinical and ICDAS. Protein microarray, real-time RT PCR, and ELISA were performed.

Results: ICAM-1, TIMP-1, TIMP-2, TNF RI, and RNF RII were consistently observed in all five sound PF. TIMP-1 and TIMP-2 showed the highest expression. TIMP-1 expression showed no significant difference between all teeth groups. TIMP-2 expression was downregulated in the symptomatic irreversible pulpitis group. TIMP2 expression in human dental pulp stem cells primed with LPS or inflammatory cytokines such as TNF-α were also explored and analyzed.

Conclusion: The expression levels of TIMPs may be implied to differentiate the inflammatory status of the pulp, and further studies will be conducted to explore the detailed role of TIMPs.