(PR49) Comparison of Soft Tissue Dissolution Capacity of Triton versus Sodium Hypochlorite

Objective: To compare the tissue dissolution efficacy of all-in-one irrigation solution (Triton, Brasseler, USA) versus 4% sodium hypochlorite(NaOCl) alone or in a mixture with 17% EDTA.

Methods: 40 4 mm diameter frozen specimens were obtained from porcine palatal mucosa. Specimens were thawed at room temperature, blotted dry and then weighed using a precision balance. They were divided into eight groups of five specimens each. Five each were immersed in 10 ml solution of Triton, NaOCl, NaOCl+EDTA and water for 20 minutes. For the remaining four groups, the samples were immersed in solution then subjected to 15 seconds sonication four times within the 20 minutes period. The samples were blotted dry and weighed again. The weight loss was calculated in the percent of the original tissue weight. Data was subjected to one way ANOVA and Tukey test for multiple comparisons (p < 0.05).

Results: Triton and 4% NaOCl solutions caused a mean weight loss of 51.78% and 51.61% in the soaking group and 65.21% and 82.19% in the sonication group respectively, with no statistical significance within the two solutions. Sonication enhanced the tissue dissolution capacity of Triton and 4% NaOCl by 1.2 and 1.6 folds respectively. However, the enhancement was only significant for 4% NaOCl (p < 0.01). Conversely, EDTA negatively impacted the dissolution capacity of 4% NaOCl with a weight loss of 11.56% (soaking) and 15.11% (sonication). Water samples had an increase in the weight.

Conclusion: Triton mixture has a similar tissue dissolution capacity to 4% NaOCl with and without sonication.