PhD student
Department of Molecular Hematology, Sanquin Research and Landsteiner Laboratory, Amsterdam, The Netherlands
Mariarosaria Miranda is an Early Stage Researcher in the EDUC8 consortium and works in the Molecular Hematology department in Sanquin (Amsterdam, The Netherlands). Her projects focuses on exploring red blood cells (RBCs) as a novel tolerogenic approach for factor VIII inhibitors employing immunodominant FVIII derived peptides presented on MHC class II. To this extend, she has used bioinformatics tools to identify promiscuously presented FVIII peptides. To supplement this data-set, she has optimized a mass spectrometry based peptide presentation assay that detects more than 4000 HLA-DR-associated peptides. Moreover, she has developed a strategy to target RBCs with FVIII derived peptides fused to a TAT cell-penetrating peptide. She has validated the TAT-FVIII peptides internalization using flow cytometric techniques. She is currently working on the assessment of RBCs phagocytosis by macrophages and whether it results in tolerogenic presentation of immunodominant FVIII peptides on MHC class II. At this ISTH conference, she is presenting a poster that shows the main FVIII derived peptides detected and the targeting of RBCs.
Monday, July 11, 2022
11:45 AM – 12:00 PM
Monday, July 11, 2022
3:00 PM – 3:15 PM