VPB0635 - Attenuation of Thrombotic Biomarkers in a Hyperlipidaemic Sprague Dawley Rats Model

Monday, July 11, 2022

6:30 PM – 7:30 PM

Location: Exhibition

Presentation Format: Virtual

Presenting Author(s)

IK

IBRAHIM K. KWAIFA

PhD Student
University PUTRA MALAYSIA
Kuala Lumpur, Kuala Lumpur, Malaysia

Co-Author(s)

KP

Kokila Vani Perumal

M. Sc. Student
University Putra Malaysia
Malaysia, Selangor, Malaysia
HB

Hasnah Bahari

Associate Professor
Universiti Putra Malaysia
Kuala Lumpur, Selangor, Malaysia
KY

Keong Y. Yong

Associate Professor
Universiti Putra Malaysia
Kuala Lumpur, Selangor, Malaysia
ZE

Zolkapli Eshak

DR.
Universiti institute Technology Mara, Malaysia
Kuala Lumpur, Selangor, Malaysia
SB

Santhra Segaran Balan S. Balan

DR.
Management and Sciences University, Malaysia
Kuala Lumpur, Selangor, Malaysia
AA

Azrina Zainal Abidin

DR.
Management and Sciences University, Malaysia
Kuala Lumpur, Selangor, Malaysia
FG

Fauzah Abd Ghadi

DR.
Universiti Putra Malaysia
Kuala Lumpur, Selangor, Malaysia
LK

Lai Shau Kong

DR.
Universiti Putra Malaysia
Kuala Lumpur, Selangor, Malaysia
SN

SABARIAH M. NOOR

Associate Professor
Universiti Putra Malaysia
Kuala Lumpur, Selangor, Malaysia

Background: Atherothrombosis is one of the major risk factors associated with the increased incidence of cardiovascular diseases, particularly in developed countries. Natural-based products intervention has been suggested as one of the essential approaches for the management of atherothrombosis-related cardiovascular diseases (CVDs). Phytochemical compounds, including gingerol, hesperidin, naringin and citric acid have been reported to exhibit antioxidant, antihyperlipidemic, anti-inflammatory, antiplatelet, anticoagulant and antithrombotic properties.

Aims: The study determined the phytotherapeutic effects of herbal extracts; a combination of Zingiber officinale (ginger), Allium sativum (garlic), Citrus Limon (lemon), Malus Domestica (Apple)/ apple cider vinegar and Honey (ZACAH) on atherothrombosis-related markers in SD rats fed with HCD.

Methods: Identification of the major related phytochemical compounds present in ZACAH extracts was performed by ultra-high performance liquid chromatography-mass spectrophotometry (UHPLC-MS) profiling at both negative and positive models. Male SD rats were randomly divided into six groups, the normal diet (ND), HCD, treatment with simvastatin (TRTSM) at 10mg/kg of body weight, treatment with ZACAH extracts (TRT1) at 1mg, 3mg (TRT2), and 5mg (TRT3) per kilogram of body weight. At the end of the 2 weeks experiments, the animals were sacrificed and the activities of thrombosis-related proteins were studied.

Results: The UHPLC-MS profiling of the phytochemicals in ZACAH extracts yielded the identification of hesperidin (retention time (RT); 11.18), citric acid (2.18), gingerol (18.62) and naringin (10.07). Oral gavage supplementation with ZACAH extracts significantly attenuated the activities of plasminogen activator inhibitor-1 (PAI-1) and tissue factor (TF) while improving the levels of tissue-type plasminogen activator (t-PA) and nitric oxide (NO) in a dose-dependent manner.

Conclusion(s): The findings suggested that identified phytochemicals available in ZACAH extracts could inhibit the progress of atherothrombotic plaque development through suppressing the expression of thrombotic marker activities induced by dietary high cholesterol intake and may represent novel therapeutic opportunities for the treatment of atherothrombosis-related CVDs.