PB1039 - Plant heparinoids and their involvement in the regulation of blood coagulation

Background: One of the urgent problems of modern medicine is the study of natural anticoagulants, the use of which does not cause adverse side effects on the organism. Many plants include heparin-like components (heparinoids), which are effective in the development of processes that prevent thrombotic complications.

Aims: The purpose of our work was to identify anticoagulant effects in different types of heparinoids isolated from the roots of peonies and to establish their regulatory effect on blood clotting processes during the development of thrombosis.

Methods: Heparinoids were isolated from the roots of herbaceous peonies (Officinalis, Ivan Goroshankin). The mechanism of ah anticoagulant action was studied and their effect on the processes of fibrin and thrombosis was investigated. Laboratory animals – male Wistar rats. Anticoagulant activity was evaluated by tests of thrombin time (TV), prothrombin time (PV), APTT and total fibrinolytic activity (TFA). Heparinoids from peonies were administered orally to rats repeatedly for 5 days every 24 hours.

Results: 20 hours after the last administration of heparinoids in the blood of animals, an increase in TV was found by 13-15%, PV - by 9-10%. APTT - by 21-24%, TFA - by 25-31%, possible mechanisms of activating action of heparinoids on anticoagulant activity of blood caused by blockade of coagulation factors. Prevention of fibrin formation processes and fibrin polymerization by heparinoids due to thrombin inhibition by plant heparinoids has been established. Thus, with hypercoagulation caused by the introduction of small doses of tissue thromboplastin, the use of heparinoids contributed to the restoration of normal blood clotting. The greatest effect was provided by the heparinoid from the roots of the Ivan Goroshankin peony. This heparinoid corresponded in molecular weight to low molecular weight heparin of animal origin.

Conclusion(s): Vegetable heparinoids having an anticoagulant effect, are able to regulate blood clotting processes, especially with hypercoagulation shifts in the bloodstream.