PB1095 - The Effect of Aqueous Chromolaena odorata (Acheampong leaf) extract use on Coagulation test parameters in Vitro

Tuesday, July 12, 2022

6:30 PM – 7:30 PM

Location: Exhibition

Presentation Format: Virtual

Presenting Author(s)

DAVID N. OFOSU, PHDc

LECTURER
DEPT OF MEDICAL LABORATORY SCIENCE (BASIC AND APPLIED BIOLOGY), UNIVERSITY OF ENERGY AND NATURAL RESOURCE
SUNYANI, Brong-Ahafo, Ghana

Co-Author(s)

AA

Akwasi Asamoah

technical instructor
Basic and applied biology dept, University of Energy and Natural Resources
sunyani, Brong-Ahafo, Ghana
RT

Ransford Tuekpe Mawuli

Undergraduate
Basic and applied biology dept, University of Energy and Natural Resources
sunyani, Brong-Ahafo, Ghana
AA

Alex Addai

Undergraduate
DEPT OF BASIC AND APPLIED BIOLOGY, UNIVERSITY OF ENERGY AND NATURAL RESOURCE
sunyani, Brong-Ahafo, Ghana
ET

Elijah Takyi Kofi

Undergraduate
DEPT OF BASIC AND APPLIED BIOLOGY, UNIVERSITY OF ENERGY AND NATURAL RESOURCE
sunyani, Brong-Ahafo, Ghana
EO

Edmond Opoku Addo

Undergraduate
DEPT OF BASIC AND APPLIED BIOLOGY, UNIVERSITY OF ENERGY AND NATURAL RESOURCE
sunyani, Brong-Ahafo, Ghana

Background: Medicinal plants, as sources of new medicines and herbal and nutritional supplements, continue to play an important role in healthcare. In Ghana, the use of Chromolaena odorata (Acheampong leaves) as a first-aid material to control bleeding is a very common practice in rural areas. The efficacy of these abundant tropical leaves has not been thoroughly assessed though it is used for diverse interventions.

Aims: This study, therefore, sought to assess the coagulation effect of aqueous extract of C. odorata (Acheampong) leaves.

Methods: In this experimental study, freshly obtained C. odorata leaves were inspected and verified by an expert ecologist. The juice from the leaves was extracted using distilled water. Blood samples were taken from 197 non-haemophiliacs who consented to participate in the study. For test samples, the extract was added to plasma whilst for paired control samples normal saline was added to the plasma. The samples were analyzed for the coagulation markers Prothrombin Time (PT) and activated partial thromboplastin time (aPTT)

Results: The study results showed that the use of aqueous C. odorata extracts significantly reduced the PT of the study participants (17.89±3.06s to 16.43±2.51s, p < 0.0001) but not the aPTT (38.83±7.13s against 39.12±7.85, p = 0.709).

Conclusion(s): C. odorata proved to be effective in promoting coagulation in vitro via Extrinsic Pathways. It is recommended that further studies could be done to identify, isolate and purify the active chemical compound in C. odorata leaves responsible for the prolonged effect on the Prothrombin Time.