PO0011 - Thrombin generation test in patients with Covid-19

Background: TGA is a sensitive and specific method for diagnosing thrombin formation and one of the best methods correlating with bleeding or thrombotic events. TGA provides information about the total clotting potential, because thrombin generation does not stop after the formation of fibrin clots .

Aims: To evaluate the parameters of the thrombin generation test in patients with Covid-19.

Methods: A retrospective study on the basis of the regional center for antithrombotic therapy of SBHI CCH "1 City Hospital named after E.E.Volosevich". Patients with confirmed Covid-19 (n=100). Blood sampling to determine the parameters of the thrombin generation (kinetics) test (TGA) was performed on the 1st day of the study. The automatic formulation of the TGA method is carried out on an open-type coagulometer — Ceveron® alpha TGA. Genetic methods - Real-time PCR, Litech Company (Russia): Hemostasis system genes: FII G20210A, FGB G455A, PAI-1 675 5G/4G.

Results: It was found that Tlag (min) and tPeak (min) significantly decreased in patients on the 1st day of hospitalization. Correlation analysis of Tlag (min) with the genotype of F I (fibrinogen) rS =- 0.3 (p=0.02), confirming that heterozygous polymorphism of the FI gene is associated with a decrease in Tlag (min). The presence of polymorphism in the PAI-1 gene is associated with a decrease in tPeak (min), i.e. in patients with polymorphism in PAI-1, the peak of thrombin is achieved faster (rS =0.5; p=0.03). The presence of polymorphism in the F I gene is associated with an increase in Peak (nmol/l), which is significantly higher in contrast to patients without polymorphism. The presence of polymorphisms in the PAI-1 and F I genes is associated with an increase in EPT (nmol/min: rS=0.3; p=0.04 and rS =0.6; p=0.02)

Conclusion(s): The results of TGA in our study reflect data on an increase in the procoagulant potential of blood in patients with COVID-19.