VPB0519 - Extracellular vesicles number and cell subtype may be influenced by diabetes mellitus and metformin in patients at high cardiovascular risk

Monday, July 11, 2022

6:30 PM – 7:30 PM

Location: Exhibition

Presentation Format: Virtual

Presenting Author(s)

PS

Paola G. Simeone

Medical Doctor , PhD Student
Department of Medicine and Aging, and Center for Advanced Studies and Technology (CAST), Via Luigi Polacchi, Chieti, Italy
Pescara, Abruzzi, Italy

Co-Author(s)

RL

Rossella Liani, PHD

Research Fellow
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Chieti, Abruzzi, Italy
GB

Giuseppina Bologna

Research Fellow
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Chieti, Abruzzi, Italy
RT

Romina Tripaldi

Research Fellow
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Chieti, Abruzzi, Italy
AD

Augusto Di Castelnuovo

Senior Researcher
Mediterranea Cardiocentro, Napoli, Italy
Napoli, Campania, Italy
PS

Pasquale Simeone

Research Fellow
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Chieti, Abruzzi, Italy
DD

Damiano D'Ardes

PhD Student
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Chieti, Abruzzi, Italy
SM

Sebastiano Miscia

Professor
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Chieti, Abruzzi, Italy
fC

francesco Cipollone

Professor
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Chieti, Abruzzi, Italy
MM

Marco Marchisio

Full Professor
G. d'Annunzio University
chieti, Abruzzi, Italy
AC

Agostino Consoli

Professor
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Pescara, Abruzzi, Italy
PL

Paola Lanuti

PA
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Chieti, Abruzzi, Italy
FS

Francesca Santilli

PA
Department of Medicine and Aging, Center for Advanced Studies and Technology (CAST), Chieti, Italy;
Pescara, Abruzzi, Italy

Background: Extracellular vesicles (EV) represent a population of small vesicles deriving from all types of cells, generated by the extroflection of the plasma membrane, and released into the circulation. EV can have both pro- and anti-atherothrombotic effects depending on the clinical setting, origin cell, stimuli, and different treatments might affect their levels.

Aims: The primary endpoint of our study was to compare the amount of circulating EV and specific EV subtypes derived from platelets, endothelial cells, and leucocytes in subject at high CV risk, with and without T2DM, and if any ongoing anti-diabetic drugs could affect the levels of EV.

Methods: Fifty-nine type 2 diabetes mellitus (T2DM) patients and forty patients without T2DM with or without prior vascular disease, were enrolled, this was a cross-sectional study, extracellular vesicles were evaluated by flow cytometry analysis.

Results: After propensity score matching the levels of total EV (Cohen d=0.55, p=0.064), total Annexin+ EV (d=0.49, p=0.043), EV CD41a+ platelet derived EV (d=0.51, p=0.014), CD41a+/AnnexinV+ platelet derived EV (d=0.50, p=0.019) and CD45 leukocyte derived EV (d=0.19, p=0.012) were lower in diabetic patients vs. those without T2DM. Interestingly, platelet-derived EV positive for AnnexinV were lower in patients on treatment with metformin (d=0.72, p=0.012).

Conclusion(s): Our study showed that, among high CV risk patients, treated with the state-of-the-art preventive strategies, T2DM is associated with lower levels of total, platelet-and leucocyte-derived EV, and that this difference may be accounted for, at least in part, by ongoing treatment with metformin, the most widely prescribed oral antidiabetic agent.