VPB1285 - The Role of Endothelial Colony-Forming Cells in Acute Deep Vein Thrombosis

Tuesday, July 12, 2022

6:30 PM – 7:30 PM

Location: Exhibition

Presentation Format: Virtual

Presenting Author(s)

Leticia Queiroz da Silva, Doctor of Science

University of Campinas
Campinas, Sao Paulo, Brazil

Co-Author(s)

SD

Silmara Aparecida De Lima Montalvão

Biologist
Hemocentro UNICAMP, University of Campinas, Campinas, SP, Brazil
Campinas, Sao Paulo, Brazil
SH

Stephany Huber

Biologist
UNICAMP
Campinas, Sao Paulo, Brazil
SS

Sandra Soares

Technician
UNICAMP
Campinas, Sao Paulo, Brazil
AL

Arlindo Lemos Junior

Physician
UNICAMP
Campinas, Sao Paulo, Brazil
OC

Otacílio Camargo-Junior

Physician
PUCC
Campinas, Sao Paulo, Brazil
CS

Claudio Simões

Physician
PUCC
Campinas, Sao Paulo, Brazil
MA

Márcia Abreu

Physician
PUCC
Campinas, Sao Paulo, Brazil
EV

Erich V. Vinicius de Paula

Professor
University of Campinas
Campinas, Sao Paulo, Brazil
JA

Joyce Annichino-Bizzacchi

Supervisor and professor
Hemostasis and Thrombosis Laboratory, Hematology and Hemotherapy Center, University of Campinas (UNICAMP)
Campinas, Sao Paulo, Brazil

Background: Deep Vein Thrombosis (DVT) is characterized by the formation of a thrombus within deep veins, and it is described as a multifactorial disease whereas the interaction of risk factors can trigger the thrombotic process. Thirty percent of DVT are classified as non-provoked DVT, and the search for new factors involved in the process is a challenge. Endothelial investigation can be an interesting point in this situation but has limitations about the anatomical access.

Aims: To evaluate the role of endothelial cells in patients with acute DVT when compared to healthy individuals.

Methods: The study was approved by a recognized medical ethics committee and after the volunteer’s consent, the blood was collected to isolate the Endothelial Colony-Forming Cells (ECFCs). Once these cells were obtained and properly immunophenotyped by flow cytometry, it was assessed the endothelial function through proliferation, apoptosis, and migration assays.

Results: Twenty-five patients were selected, however, only 5 had their ECFCs successfully isolated – a group of study. Interestingly, all ECFCs patients were non-provoked DVT (n=3) or associated with minor risk factors (n=2). The median age of the DVT group was 55 years against 33 years of the control group, the other clinical and descriptive data are shown in table 1. Regarding the ECFS, the morphology was similar in both groups (table 1), however, the ECFCs isolation was a challenge in DVT patients, with 20% of success vs. 50% in controls. The endothelial data through automated live cell analysis (Incucyte®) evidenced increased endothelial cell death in DVT compared to the controls, significantly at 24 hours of experiment, table 2. In proliferation and wound healing assay, despite variations between the medians, it was not found statistical significance among the comparations, probably due to the small sample size of DVT patients.

Conclusion(s): Our data suggest an alteration of endothelial cells in acute DVT.