Stanford University
Nam Soo Joo, Ph.D. Senior Research Scientist at CF translational research lab at pediatrics department/Stanford university.
I have been working in CF research field with a special emphasis on a potential role played by airway submucosal glands in the process of CF lung disease since I joined the cystic fibrosis research laboratory, directed by Dr. Jeffrey J. Wine, at Stanford in 1999. Since then, I have contributed to the development of important methods in collaboration with colleagues at Stanford: an in situ optical gland secretion assay for measuring fluid secretion rates and compositions from individual airway submucosal glands; a bioluminescence assay for bacterial killing with saliva samples; proteomic assay of gland mucus from CF and control human airways; airway smooth muscle contraction assay & and mucociliary clearance (MCC) assay for measuring a mucociliary clearance velocity using ex vivo WT & CF animal tracheae. With these methodologies, we discovered defects in submucosal gland secretory functions in CF airways from human and CF animal models. Recently we uncovered synergistic MCC in ex vivo animal tracheae by combined β adrenergic and low dose cholinergic agonists, including CF ferrets without inducing airway narrowing. To better understand the underlying mechanisms of synergistic enhancement of MCC and to advance preclinical work, experiments are underway using ex vivo & in vivo MCC assay, pH stat experiments, airway surface liquid (ASL) depth measurement, dynamic light scattering microrheology assay, and in situ gland secretion assay with WT & CF animals. I recently transferred to pediatrics department (joined Dr. Carlos Milla lab) and continue our CF transitional research.
Saturday, November 5, 2022
9:45 AM – 11:45 AM ET
Saturday, November 5, 2022
3:30 PM – 5:30 PM ET