Principal Research Scientist Abbvie, Illinois, United States
High-throughput screening (HTS) is a crucial step in drug discovery. Traditional HTS assays rely on UV/Vis or fluorescence spectroscopy for detection. These detection methods are rapid and sensitive, but sometimes limited by their specificity and are prone to optical artifacts from compounds. Mass spectrometry (MS) on the other hand, can provide high sensitivity and specificity for biological assays. However, its use had been limited to lower throughput assays due to the use of chromatography to overcome ion suppression from the sample matrix. We have adapted and applied IR-MALDESI-MS for high throughput ( < 1 sample/sec) biochemical and cellular assays and protein analysis. MALDESI-MS allows direct ambient analysis of complex reaction mixtures in common biochemical buffers and cellular medias without sample clean-up. Examples of cellular and biochemical assays as well as protein analysis will be given along with other potential applications including in the ADME space.
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