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Automation Technologies

Robotic Automation of Organoid Culture and Analysis

Primary Author - January Poster(s)

SM

Sarah Moss, MS

Scientist II
Advanced Solutions Life Sciences
Manchester, NH, United States

Abstract: Tissue organoids, or “mini organs” hold great potential as tools for high throughput analysis, due to their small size and consequently low cost. Despite this, organoids are typically fabricated and cultured manually “by-hand”, limiting their effectiveness as a high throughput tool. In the present study, we leveraged our state-of-the-art automation BioAssembly™ platform, including the BioAssemblyBot® (BAB) 6-axis robotic system, BioStorageBot™ incubator, and an intuitive BioApps™ workflow control software, to implement a fully automated workflow for culture and analysis of colorectal cancer organoids (Cellesce). A key advantage of this system is its flexibility. Due to our wide array of tools, we have the capacity to fabricate organoids from a wide range of materials and can easily accommodate any size well plate. The automated workflow entailed accurately dispensing 7ul droplets of chilled organoid/Matrigel suspension into each well of a 96 well plate. Media is then added to each well and the plate transferred to the modular incubator. Temperature control within the stage under the well plate ensured rapid Matrigel gelling after dispending droplets. The time to dispense the Matrigel/organoid domes into a 96-well plate (minus the edge wells) was 5.5 minutes, which is half the time it takes a human to complete the same task. During culturing, media was changed regularly using a custom tilted stage to enable complete medium removal at each step without disrupting the domes. At the end of the culture duration, culture medium was removed and, following a staining procedure, the plate was transferred into a confocal scanner and automatically imaged using a pre-built scanning protocol. The scanner is designed for high throughput analysis and can take multiple confocal stacks each well of the 96 well plate without any human input. This entire workflow was controlled by our BioApps™ software and executed by the BioAssembly™ platform, limiting human involvement to only the initial loading of materials into the platform. In ongoing experiments, we are using this compact, all-in-one robotic automation platform to assay organoid responses, via rapid high-content analysis to different pharmaceuticals. Using this automation solution is not only conducive to higher throughput, experiments with more complex tissue models, but also reduces the risk of human error in performing the experiments. Such workflows could accelerate identification and development of new, potentially life-saving therapeutics

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Each participating poster in the exhibit hall will have a QR code next to it. For virtual participants, look for the scavenger hunt icon for participating posters.