CyBio FeliX: Making Simple Western™ Technology … more simple

The Simple Western™ JESS is a capillary based automated Western blot instrument capable of screening 24 samples from generation to results in approximately 5 hours. This time saving and throughput is advantageous compared to traditional Western blot systems that take 24 to 48 hours to generate results. The error prone steps of traditional Western blotting are eliminated but automating the protein separation and immunodetection process, then the associated software quickly processes the quantifiable data. At Aurelia Bioscience, the Simple Western™ JESS is a staple technique in a repertoire of drug discovery methodologies. Our aim was to use the CyBio FeliX to further simplify the Simple Western™ procedure, allowing inexperienced users to access the technology while also increasing the reproducibility of JESS data. In order to examine the capabilities of the technology, THAL-SNS-032 (a well-studied PROTAC) was examined for its ability to degrade CDK9, then compared with data previously generated for the Protein-Simple™ webinar “Advancing Targeted Protein Degradation Research with Automated Western Blotting”.