Research Associate The Broad Institute Cambridge, Massachusetts, United States
Abstract: PRISM is a cutting-edge technology for multiplexed cancer cell line viability screening to evaluate anti-cancer molecules or other perturbagens in hundreds of cancer models simultaneously. Cell lines are stably infected with unique DNA barcodes, pooled, and then co-treated for 5 days. Cells are then lysed for PCR amplification and barcode detection. Using automated liquid handlers and Luminex FlexMAP 3D technology, mRNA is captured from lysate, reverse transcribed, amplified, and detected. PRISM has adapted the Luminex detection platform’s 500 polystyrene beads by coupling to complementary oligonucleotides that are then excited by lasers to measure the PRISM barcode abundance. Using relative barcode abundance allows for the generation of cell line sensitivity signatures by comparing treatment conditions to control conditions. The PRISM sensitivity profiles are used in conjunction with genomic information to generate predictive models to identify features that correlate with sensitivity.
Scan and Win! Complete as many scavenger hunt questions as possible. You will find scavenger hunt QR Codes to scan on participating posters. Each participating poster in the exhibit hall will have a QR code next to it. For virtual participants, look for the scavenger hunt icon for participating posters.
SLAS Events
Instructions
Each poster participating in the scavenger hunt will have a SCAVENGER HUNT QR code. When that QR code is scanned using the SLAS2022 app, question about the poster will appear. You must answer the question correctly to collect points. BE SURE TO EXPLORE THE POSTER BEFORE SCANNING THE CODE! SLAS will award three prizes to the participants with the most points.
1st Prize - Comp Reg + Hotel/Airfare to SLAS2023 in San Diego
2nd Prize - $50 Starbucks Gift Card
3Rd Prize - $25 AMEX Gift Card
Keep an eye on the leader boards to see who’s at the TOP. Winners will be announced after SLAS2022.
Each participating poster in the exhibit hall will have a QR code next to it. For virtual participants, look for the scavenger hunt icon for participating posters.
