Rapid fieldable biothreat identification via laser-based PCR

Real-time fieldable detection of pathogens has long been the goal of biosensor technologies for use by first responders and other front-line personnel. Laser-based plasmonic PCR is a promising technique for miniaturizing traditionally laboratory-stationed PCR systems. Thermocycling PCR reagents with a compact laser system instead of a conventionally heated thermal block lowers the size and weight of the system, and enables much more rapid thermocycling for near real-time detection. We have prototyped a tabletop system that fits on a small optical breadboard. Our system provides a path for further miniaturization, with the possibility of deploying it on mobile platforms in the field. We believe this opens up the opportunity for automated sample collection, sample preparation, and system operation that will allow end-users to run PCR assays on biological targets at a rapid pace in a fielded situation, and to do so without requiring extensive laboratory training beforehand.