Professor American University of Sharjah Sharjah, Sharjah, United Arab Emirates
Abstract: Cardiovascular diseases (CVDs) are the leading causes of death in intensive care units worldwide, particularly in UAE and Mediterranean countries. The World Health Organization stated that by 2030, almost 23.6 million people will die from CVDs. The buildup of plaque and blockage of arteries leads to potentially deadly acute myocardial infarction (MI), known as “heart attack”. Electrocardiography (ECG) is one of the common diagnostic tools for spotting and triaging MI patients however more than 40% of MI cases show normal ECG profile while testing. The measurement of certain biomarkers in the patient blood could provide a more accurate diagnosis of MI. Cardiac troponin I (cTnI), the central key element of cardiac muscle regulation and contraction, is considered the standard biomarker for the diagnosis of MI. This study reports the development of an efficient fluorescent immunoassay for the detection and quantification of cTnI using liposome-based fluorescent signal amplification. The assay is based on capturing cTnI molecule between a surface-immobilized capturing antibody and a biotin-conjugated secondary antibody (detection antibody). Signal development was achieved by subsequent addition steps of streptavidin and biotin-conjugated liposomes which are loaded with a fluorescent dye. The fluorescent signal is greatly enhanced upon the release of loaded dye from the liposomes.The standardized assay is sensitive and selective towards cTnI both in physiological buffer solutions and human serum samples and provides a wide linear dynamic range relevant to clinical concentrations.
Scan and Win! Complete as many scavenger hunt questions as possible. You will find scavenger hunt QR Codes to scan on participating posters. Each participating poster in the exhibit hall will have a QR code next to it. For virtual participants, look for the scavenger hunt icon for participating posters.
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Instructions
Each poster participating in the scavenger hunt will have a SCAVENGER HUNT QR code. When that QR code is scanned using the SLAS2022 app, question about the poster will appear. You must answer the question correctly to collect points. BE SURE TO EXPLORE THE POSTER BEFORE SCANNING THE CODE! SLAS will award three prizes to the participants with the most points.
1st Prize - Comp Reg + Hotel/Airfare to SLAS2023 in San Diego
2nd Prize - $50 Starbucks Gift Card
3Rd Prize - $25 AMEX Gift Card
Keep an eye on the leader boards to see who’s at the TOP. Winners will be announced after SLAS2022.
Each participating poster in the exhibit hall will have a QR code next to it. For virtual participants, look for the scavenger hunt icon for participating posters.
