Chronic paclitaxel (PTX) treatment causes excruciating pain in cancer patients, limiting its use in cancer chemotherapy. Herein, neuroprotective potential of synthetic cannabidiol (CBD) and CBD formulated in extracellular vesicles (CBD-EVs) isolated from human umbilical cord derived mesenchymal stem cells were studied against PTX-induced neuropathic pain (PIPN) in C57BL/6J mice. EVs and CBD-EVs particle size, surface roughness, nanomechanical attributes, stability, and release studies were investigated. CBD-EVs treatment significantly improved mechanical and thermal hypersensitivity (P < 0.001) as compared to EVs or CBD alone. PTX-treated mice's dorsal root ganglions and spinal homogenates had mitochondrial dysfunction which was significantly improved by CBD and CBD-EVs by regulating the AMPK pathway (P < 0.001). Blocking studies with 5HT1A receptors and AMPK demonstrated that CBD had no effect on PIPN neurobehavioral or mitochondrial function. Our results suggest that CBD-EVs can be a novel therapeutic option for the treatment of PIPN and CBD treatment activates AMPK axis in regulating PIPN
Learning Objectives:
Upon completion, participants will be able to understand the role of Exosomes (EVs) in Taxol induced neuropathy.
Upon completion, participants will be able to learn protocols about separation of extracellular vesicles(EVs) from human umbilical cord mesenchymal stem cells
Upon completion, participants will be able to learn the various behavioral parameters used in chemotherapy induced neuropathy
Upon completion, participants will be able to understand the role of cannabinoids in pain especially chemotherapy induced neuropathy
The participants would be able to have a idea as to the possible mechanisms by which cannabinoids have role in pain
