A novel semi-automated microsampling approach utilizing Tasso-M20 provided a reliable and convenient technique for at-home sample collection and was first used in one of our clinical programs. To adopt Tasso-M20 for broader clinical use, we characterized the Tasso-M20 sorbent using nine model compounds by leveraging our experience with Volumetric Absorptive Microsamping (VAMS). Potential compound matrix instabilities on Tasso-M20 were investigated in EDTA and sodium heparin blood. Additionally, clinical sampling deviations and storage variations were assessed, and an automation workflow was explored.
Following a high-level standard approach for feasibility assessment for Tasso-M20, all nine compounds evaluated achieved acceptable precision and accuracy, recovery, and minimum 1 week stability at RT and 40℃/75%RH. Five out of seven VAMS methods were directly transferable to Tasso-M20 method, while two required major modifications.
The evaluation has demonstrated the feasibility of using Tasso-M20 to support regulated bioanalysis for clinical samples.
Learning Objectives:
Upon completion, participant will be able to recognize the opportunity to conduct Tasso-M20 microsampling in their clinical studies and develop a Tasso-M20 method following the approaches recommended.
Upon completion, participant will be able to confidently transfer their VAMS method to a Tasso-M20 method utilizing a decision tree, to enable the microsampling with the user-friendly device at home.
Upon completion, participant will be able to set up an automated workflow to improve the throughput and capacity while improving assay reproducibility and reducing ergonomic related injuries.
