Liposomes have received renewed interest not least due to the successful application of lipid nanoparticle based delivery of mRNA vaccines in the context of Covid-19. However, liposome preparations are still lacking in scalability and encapsulation efficiency (EE). In this presentation we describe a novel one pot – one step process to formulate final liposome preparations in the nanometer size range, a PDI below 0.2 with an EE of up to 60%. The technology will be described in general and a case study using a model peptide will be presented, including dissolution data in Simulated Intestine Fluid (SIF).
Learning Objectives:
To understand the concept of this new technology
To understand the suitability and possibilities of this technology for the delivery of specific macromolecules, PROTAC´s and small, low soluble molecules for future developments
To understand the characterization of the NLS, the incoporation of the different drugs into the liposomal matrix and the celluar up-take
