Doctoral Candidate St. John's University Jamaica, New York
In this presentation, the anticancer activity of Nisin ZP, an antimicrobial peptide, in non-small cell lung cancer (NSCLC) cells and formulation development into dry powder to enable inhaled delivery will be demonstrated. Nisin showed selective toxicity in NSCLC (A549 and H1299) cells and confirmed the non-membranolytic activity of cancer inhibition by ROS-induced apoptosis, cell-cycle arrest, and mitochondrial membrane depolarization. Further, nisin inhibited cancer cell migration and exhibited excellent antitumor potential in vitro. Nisin was spray-dried with mannitol, L-leucine, and trehalose in an optimized ratio to obtain dry powder for inhaled delivery. Nisin spray-dried powder (NZSD) showed good yield and drug loading, semi-crystalline nature, and spherical morphology. Aerosolization performance revealed aerodynamic diameter < 3 µm and a high fine particle fraction (~80%) suitable for powder deposition in respiratory airways of the lungs. In addition, NZSD powder maintained inhibitory potential and was stable for 3 months at room temperature and refrigerated storage conditions.
Learning Objectives:
Upon completion, the participant will be able to demonstrate the use of antimicrobial peptides for cancer therapeutics.
Upon completion, the participant will be able to conduct spray drying of proteins and peptides to improve the stability and delivery potential.
Upon completion, the participant will be able to describe the need for inhaled delivery of therapeutics to target cancer at a local site in the lungs.
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